Digested Plasmid Clean Up

Add equal volume water Add equal volume (including the water) Phenol:Chloroform, vortex well Spin for 10 min at 13.8K rpm (18K rcf) Save supernatant in new tube Add 10% volume 3M Sodium Acetate Add 250% volume 100% EtOH, vortex well Place in -20° for 30min Spin for 30min Discard supernatant Re-suspend in 70% EtOH Spin for 5 min Suck off most of the supernatant, and dry in vacuum centrifuge (It is ok to let DNA dry completely) Re-suspend in 30ul water