- 1st Strand Synthesis
- Agarose Gel Preparation
- Bisulfite Protocol
- CRISPR/Cas9 for Glycine Max
- Common Solutions
- Constructing Coda ZFNs (.pdf)
- Digested Plasmid Clean Up
- Ligation
- Metaphase Chromosome prep (NO2)
- Nano Drop
- Plasmid Digestion
- Plasmid Preparation
- Primers
- Promega Maxi Prep
- Protocol for Chromosome Squashing
- Rescuing a Band from a Gel
- Root Cross Section Chromosome Squash
- Soybean Hairy Root Transformation
- Soybean Transformation Half-Seed Explant
- Standard Ligation Reaction
- Standard PCR protocol
- Thermal Assymmetric Interlaced PCR (.pdf)
- ZFN Creator (.pdf)