Root Cross Section Chromosome Squash

Equilibrate root in 45% acetic acid.

Cut off root cap with a razor blade.

Cut off a somewhat thin cross sectional slice, put on slide with a cover slip and a drop of acetic acid.

Slide the razor under one corner of the cover slip and gently heat over a flame.

Tap a few times with the back of a probe and flame again.

Tap more times in a circular pattern.

Cover with filter paper and lightly press with thumb.